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Protein deubiquitination is becoming increasingly
2020-05-14

Protein deubiquitination is becoming increasingly instrumental in understanding the complexities of the Ub system. Adding to this complexity is the discovery of bacterial effectors with DUB activity that have structurally adapted themselves to interfere with the eukaryotic Ub system (Pruneda et al.,
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Elevation of plasma glucocorticoids as well as a sympathetic
2020-05-14

Elevation of plasma glucocorticoids as well as a sympathetic activation are highly adaptive mechanisms allowing the individual to cope in various situations [39]. Novelty-induced increase in locomotor activity, characterizing the bold fish herein, accompanied by elevated glucocorticoids is a well-es
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Saururus chinensis Lour Baill Saururaceae a perennial
2020-05-14

Saururus chinensis (Lour.) Baill. (Saururaceae), a perennial herb, is widely cultivated in China and southern Korea. It has been traditionally used as folk medicine for the treatment of inflammation, jaundice and gonorrhea [8]. Previous chemical studies of S. chinensis have revealed the presence of
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While lowering the TID exposure of electronics is fairly
2020-05-13

While lowering the TID exposure of electronics is fairly straightforward, there are other Cladribine induced effects that are not as easily mitigated, especially with COTS components. Single Event Effects (SEE) occur when a high-energy particle strikes the active area of an integrated circuit, caus
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br Introduction Identification and correct storage of semina
2020-05-13

Introduction Identification and correct storage of seminal stains are crucial for the investigation of sexual assault cases. Actually, undetected ‘cold’ cases involving sexual assault can be solved decades after investigations analysing DNA from stored evidences [1]. Recently, STR analysis and th
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Moreover maintaining the pyrimidine pool is not the
2020-05-13

Moreover, maintaining the pyrimidine pool is not the only important role played by DHODHs. In Toxoplasma gondii, for instance, DHODH plays a second essential function [38] possibly coupled to the mitochondrial respiratory activity, where it replenishes ubiquinol levels and prevents reactive oxygen s
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br Experimental All starting materials
2020-05-13

Experimental All starting materials and reagents were purchased from Sigma-Aldrich, Merck and Loba Chemie. Thin layer chromatography (TLC) was performed on silica gel G60 F254 (Merck) plates and eluted with the mobile phase n-hexane: ethyl acetate (70:30%v/v). Melting points were recorded on auto
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Currently two approaches can be used
2020-05-13

Currently, two approaches can be used individually or combinedly to overcome weak immunogenicity of subunit Viomycin (Bookstaver et al., 2018; Moyle, 2017; Vartak and Sucheck, 2016). The first is to design particulate antigens. Virus-like particles (VLPs), for example—mainly derived from the capsid
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The importance of AAE in enhancing the
2020-05-12

The importance of AAE3 in enhancing the survival of plants and yeast when confronted with certain environmental stresses has been documented in recent reports. In response to biotic stress such as oxalate-secreting micro-organisms, AAE3 was found to reduce the inhibitory growth effects of the secret
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TgTCEA from petals TgTCEA and TgTCEB from
2020-05-12

TgTCEA from petals (TgTCEA1) and TgTCEB from pollen grains (TgTCEB1) were used throughout the study. The recombinant enzymes were expressed in , and purified as the His-tag-free forms as described previously., Enzyme reactions were performed at 4 mM substrates under the same conditions as described
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How does an increase in E affinity for
2020-05-12

How does an increase in E2 affinity for the RING domain favor higher activity and polyubiquitination by the E2? Binding of the RING domain to the E2 positions the donor Ub in the E2~Ub conjugate in a closed complex [16], [17], [18] that increases the reactivity of the thioester [60]. A higher affini
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5 aminosalicylic acid br Materials and methods br Results
2020-05-12

Materials and methods Results Discussion Plant U-box E3 Ub ligases regulate diverse plant-specific cellular processes [1], [2], [3], [4]. However, structural and functional relationships between U-box E3s and their E2 partners are largely unknown in higher plants [24], [25]. In this study,
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br Development of the double
2020-05-12

Development of the double strand break repair model for meiotic recombination Meiotic recombination was first described by Frans Alfons Janssens using Polyphyllin VII in the salamander Batracoseps attenuatus in 1909 [21] and then further elaborated at the genetic level in Drosophila by Thomas H.
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A topological property P is
2020-05-12

A topological property P is said to be cut point hereditary [3] if whenever a space X has property P, then, for every there exists some separation of such that each of and has property P. A space X is called connected ordered topological space (COTS) [5] if it is connected and has the property
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Materials and methods Construction of
2020-05-12

Materials and methods Construction of the Chk-YB-1b-Neo vector. Approximately 107 plaques from a chicken genomic library established in EMBL3 vector (Clontech Palo Alto, CA) was screened and one clone containing a 12kb insert was selected. Digestion of this clone with SalI and EcoRI restriction enz
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